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Figure 5 | Cancer Cell International

Figure 5

From: Sensitization of U937 leukemia cells to doxorubicin by the MG132 proteasome inhibitor induces an increase in apoptosis by suppressing NF-kappa B and mitochondrial membrane potential loss

Figure 5

Assessment of senescence in U397 human leukemic cells treated with MG132 (1 μM), Doxorubicin (DOX) 1 μM, or MG132 + DOX. U937 cells (1 × 106) were incubated alone or with different treatments for 24 h at 37°C in a humid atmosphere containing 5% CO2 and 95% air in RPMI-S medium. After incubation, the cells were washed in the same medium, and senescence was determined by measuring SA-β-gal utilizing flow cytometry. For each sample, at least 20,000 events were acquired in a FACSAria-I cell sorter and the data were analyzed with FACSDiva software. Results are shown as % and represent the mean ± the Standard deviation (SD) of three independent experiments performed in triplicate. Statistical analysis, the Mann–Whitney U test. *p <0.05 MG132 + DOX vs DOX; ♦p <0.05 DOX vs all groups. UCG = Untreated control group.

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