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Table 1 Primer pair used for real-time quantitative PCR

From: Sensitization of U937 leukemia cells to doxorubicin by the MG132 proteasome inhibitor induces an increase in apoptosis by suppressing NF-kappa B and mitochondrial membrane potential loss

Gene Primer pair sequences Gen Bank Accession No.
NOXA 5'GAC ATG CCT GGG AAG AAG G3' NM021127
  5'TCC TGA GCA GAA GAG TTT GGA 3'  
BAX 5'TTT GCT TCA GGG TTT CAT CC 3' NM138764
  5'CAG TTG AAG TTG CCG TCA GA 3'  
DIABLO 5'TGA CTT CAA AAC ACC AAG AGT A3' NM019887
  5'TTT CTG ACG GAG CTC TTC TA 3'  
DR4 5’CTC GCT GTC CAC TTT CGT CTCT3’ NM003844
  5’GTC AAA GGG CAC GAT GTT3’  
FAS 5’TGA ACA TGG AAT CAT CAA GGA3’ NM000043
  5’CAA AGC CTT TAA CTT GAC TT3’  
BCL-XL 5'GCA GGC GAC GAG TTT GAA CT 3' NM138578
  5'GTG TCT GGT CAT TTC CGA CTG A 3'  
MCL-1 5'CAC GAG ACG GTC TTC CAA GGA TGC T 3' NM021960
  5'CTA GGT TGC TAG GGT GCA ACT CTA GGA 3'  
SURVIVIN 5'TGA GCT GCA GGT TCC TTA TCT G 3' NM001168
  5'GAA TGG CTT TGT GCT TAG TTT T 3'  
IkBa 5'GGA TAC CTG GAG GAT CAG ATT A 3' NM001278
  5'CCA CCT TAG GGA GTA GTA GAT CAA T 3'  
P65 5'GCA GGC TCC TGT GCG TGT CT 3' NM02975
  5'GGT GCT CAG GGA TGA CGT AAA G 3'  
L32 5'GCA TTG ACA ACA GGG TTC GTA G 3' NM000994
RIBOSOMAL 5'ATT TAA ACA GAA AAC GTG CAC A 3'  
PROTEIN   
  1. The oligonucleotides were designed using Oligo ver.6 software. Gene sequences were obtained from the GenBank Nucleotide Database of the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov).