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Figure 3 | Cancer Cell International

Figure 3

From: Wnt5a promotes migration of human osteosarcoma cells by triggering a phosphatidylinositol-3 kinase/Akt signals

Figure 3

Wnt5a promotes cell migration via Akt pathway. (A) Human osteosarcoma cells MG-63, serum-deprived for 24 h, were untreated or treated with 100 ng/ml of Wnt5a and harvested at 15 min, 30 min, and 1 h after the start of treatment for SDS-PAGE and immunoblot analysis with antibodies to p-Akt (p-Ser473) and total Akt. p-Akt/total Akt ratios were indicated at each time. Data were presented as mean ± SD of 3 determinations. *, **: p < 0.05, p < 0.01 in the cultures with Wnt5a relative to the cultures without Wnt5a. (B) An equal number of MG-63 cells were pre-treated with 20 μM LY294002 (PI3K inhibitor) for 1 h, then incubated with 100 ng/ml Wnt5a for 15 min. After stimulation, cells were analyzed by immunoblotted with p-Akt (p-Ser473) or total Akt antibodies. Data were presented as mean ± SD of 3 determinations. **, ##: p < 0.01, p < 0.01, in the cultures with Wnt5a relative to the cultures without Wnt5a.

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