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Figure 6 | Cancer Cell International

Figure 6

From: An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus

Figure 6

Intracellular localization of PTI-1 protein. V5-tagged PTI-1 protein was transiently expressed in NIH 3 T3 cells from the constructs pcDNA3.1-PTI-1-ORF1, pcDNA3.1-PTI-FL and pcDNA3.1-PTI-1-FL-ΔATG. The cells were fixed and stained with mouse anti-V5 antibody and Alexa-fluor® 488 goat anti-mouse antibody (green) and the DNA stain TO-PRO-3 (red). (A) Images recorded by CLSM using a x100 oil-immersion objective showing the localization of V5-tagged PTI-1 protein and nuclear DNA. (B) Scoring of the cellular distribution of PTI-1 protein. The transiently transfected cells were observed under a fluorescent microscope. The assay was repeated three times with three independent transfections with each construct. Over 100 cells were scored each time for each construct transfection. The cells were assigned to one of three categories: mainly nuclear localization (red bars), mainly cytoplasmatic localization (green bars) or even distribution between the nucleus and the cytoplasm (yellow bars). Standard deviations are shown.

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