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Figure 5 | Cancer Cell International

Figure 5

From: Par6 is an essential mediator of apoptotic response to transforming growth factor beta in NMuMG immortalized mammary cells

Figure 5

Par6/wt-overexpressing cells maintain a high apoptosis response to TGFβ in long-term treated 3D culture. 12–day old 3D structures of the indicated NMuMG-derived cell lines were treated with control media (DMSO alone), TGFβ1 (TGFβ), the TβRI/Smad2 inhibitor SB-431542 (SB) or TGFβ + SB for 144 hours, fixed and immunostained for cleaved caspase-3 (CC3, green). Dapi was used to visualize the nuclei. A. Confocal images show representative structures for each given cell line and treatment. Scale bar = 20 μm. B. Average percentage of apoptotic structures and error bars (SD) were calculated for n = 3 technical replicates within one experiment.

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