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Figure 8 | Cancer Cell International

Figure 8

From: Par6 is an essential mediator of apoptotic response to transforming growth factor beta in NMuMG immortalized mammary cells

Figure 8

Par6 and TGFβRI activation mediate TGFβ-induced loss of basal α6 integrin expression in NMuMG 3D structures. 12-day old 3D cultures of the indicated NMuMG-derived cell lines were treated with control media (DMSO alone), TGFβ1 (TGFβ), the TβRI/Smad2 inhibitor SB-431542 (SB) or TGFβ + SB for 48 hours, fixed and immunostained for α6 integrin (green). Dapi was used to visualize the nuclei (blue). A. Confocal images show representative structures for each given cell line and treatment. Scale bar = 20 μm. Arrow = apoptotic nuclei. B. Average percentage of structures expressing basal α6 integrin were quantified for n = 3 biological replicates. Two-way ANOVA for all cell lines and treatments was significant (p < 0.01). Bonferroni post test compared differences between control and treatment for each cell line, *p < 0.05, **p < 0.001.

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