Figure 6

Metastatic and invasive abilities of tumor-retrieved cells. (A, B) Representative H&E stainings of mouse lung biopsies. (A) Subjects from the Non-Rec group were sacrificed at day 49, and no metastatic areas were found in the lungs. (B) Obvious tumor metastases were found in the lungs of spontaneously deceased subjects in the Rec group, as marked by the yellow dotted lines. (C) The survival curve demonstrated that 2 subjects were lost due to poor prognosis and lung metastasis, and the remaining subjects were sacrificed at day 49. The tumors from all subjects were excised at day 14 (primary, black dotted line). (D, E) Representative phase contrast images of the matrigel invasion assay. The Non-Rec group (D) had fewer invading cells than the Rec group (E). (F) Quantification of the invasion assay results. The difference between the 2 groups is statistically significant. Scale bars represent 100 μm and 20 μm in the H&E and invasion images, respectively. *, p < 0.05.