Figure 2

Silencing of ETS1 reduces the mRNA and protein expression of ETS1 and MDR1 in MCF-7/ADR cells. (A) RT-PCR analysis and (B) quantification of ETS1 and MDR1 mRNA expression in: ETS1 siRNA-transfected cells (ETS1 siRNA); control untransfected cells (Control); and negative control siRNA transfected cells (NC siRNA) at 48 h after transfection. GAPDH was analyzed in parallel as a loading control; the ratio of ETS1 to GAPDH mRNA was calculated for each group. Western blot analysis (C and E) and quantification (D and F) of ETS1 (C and D) and MDR1 (E and F) protein expression in: ETS1 siRNA-transfected cells (ETS1 siRNA); control untransfected cells (Control); and negative control siRNA transfected cells (NC siRNA) at 48 h after transfection, β-actin was analyzed in parallel as a loading control; the ratio of ETS1and MDR1 to β-actin protein was calculated for each group. Values are mean ± SD of three independent experiments; *P < 0.05 and **P < 0.01 compared with untransfected control cells.