Figure 4
From: Silencing of ETS1 reverses adriamycin resistance in MCF-7/ADR cells via downregulation of MDR1
Rhodamine 123 (Rh123) efflux assay. (A) MDR1-mediated rhodamine efflux was measured by flow cytometric analysis of Rh123 intracellular accumulation. Flow cytometry analysis and (B) mean fluorescence intensity of control untransfected MCF-7/ADR cells (Control); control untransfected MCF-7/ADR cells treated with Rh123(control +Rh123); negative control siRNA transfected MCF-7/ADR cells treated with Rh123(NC siRNA + Rh123); and ETS1 siRNA-transfected MCF-7/ADR cells treated with Rh123(ETS1 siRNA+ Rh123). Results are mean ± SD of three independent experiments. *P < 0.05 compared with MCF-7/ADR cells treated with Rh123; # P < 0.01 compared with untransfected MCF-7/ADR cells.