Decrease of mitochondrial cytochrome c level and class III ß-tubulin by PEITC. Graph 2A shows mitochondrial cytochrome c levels after the LNCaP cells exposed to 8 μM of PEITC for 4 or 18 hours. □ indicates the background FITC-fluorescence level, in arbitrary log unit, which were from cells stained with an isotype control instead of the cytochrome c antibody. ▒ indicates the cytochrome c level in mitochondria of untreated LNCaP cells, which was a mean of the LNCaP cells cultured for 4 and 18 hours. ▧ indicates a reduced FITC-cytochrome c level in mitochondria after the cells were exposed to 8 μM PEITC for 4 hr, and ▤ indicates the cytochrome c level at 18 hr. Vertical bars indicate the ranges of the three highest peaks of fluorescence obtained in each experimental condition. Western blot in Figure 2B shows the down-regulation of the mitochondrial class III ß-tubulin protein expression in the isolated mitochondria preparation from the LNCaP cells, which were treated with 8 μM PEITC for 18 hours. The mitochondria preparation isolated from untreated LNCaP was used as a control for comparison. The levels of ß-actin were assayed as a loading control.