Figure 3

Disruption of mitochondrial membrane potential. The LNCaP cells were incubated without or with 4 μM or 8 μM of PEITC for 18 hrs. The cells were then stained with JC-1 dye to measure the membrane potential with a flow cytometric method. Separate control cell cultures were stained with CCCP, a reagent known to disrupt the mitochondrial membrane potential that shifts the untreated cells with red fluorescence to the potential disrupted cells with green florescence. The figure shows that after PEITC exposure there was a concentration-related shift of the LNCaP cells with the red fluorescence to green color as that seen with the control CCCP for membrane potential disruption. (□) indicates the percent LNCaP cells with red fluorescence, and (▐ ) indicates the percent of LNCaP cells with the green fluorescence. The vertical bars indicate the means and ranges of two independent experiments.