Figure 3
Ibrutinib down-regulated CCL3 and CCL4 gene expression in GCB-DLBCL cell lines. SU-DHL-16 and LY7 cells (1 × 105/ml) were treated with a concentration of 10 μM of ibrutinib for 12 hours. Then total RNA was extracted by TRIzol reagent and performed a reverse transcription. RealTime-PCR analysis was done to determine the quantities of CCL3 and CCL4 mRNA. β-actin was used as the internal control gene for normalization. The expression levels of CCL3 and CCL4 of SU-DHL-16 cells after ibrutinib treatment were shown in Figure 3A and Figure 3B. The expression levels of CCL3 and CCL4 of OCI-Ly7 cells after ibrutinib treatment were shown in Figure 3C and Figure 3D. Results were expressed as the mean ± SD of three independent experiments. The differences in groups were assessed by Student’s t-test (p < 0.05).