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Table 2 Clonogenicity of RPMI 8226 cells

From: Epigenetic alteration by DNA-demethylating treatment restores apoptotic response to glucocorticoids in dexamethasone-resistant human malignant lymphoid cells

Starting cells/well 10 1 10 1
  Cloning efficiency Viable cells/well
  (% wells w viable cells) (×10-5) ± SD
Control 100 50 6.0 ± 1.3 0.15 ± 0.3
Dex 100 75 6.9 ± 1.3 1.2 ± 1.5
AZA 100 50 0.7 ± 0.6 0.06 ± 0,1
AZA + Dex 75 0 0.14 ±0.1 0
  1. Clonogenicity of RPMI 8226 cells is reduced by treatment with AZA followed by Dex. RPMI 8226 cells were incubated with 100 nM AZA or DMSO vehicle for 40 hours before addition of ethanol vehicle (control) or 1 μM Dex for an additional 96 hours. Cells were then serially diluted to initial staring densities of 100, 10, or single cells in cloning medium. Cells from independent wells for each treatment were counted using a Trypan blue exclusion assay 2 weeks after cloning. Shown are cloning efficiency, range and sum of viable cells from all wells seeded with 10 or single cells for each treatment. Data in bold emphasize the AZA+Dex effect.