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Figure 1 | Cancer Cell International

Figure 1

From: Simulating tumor microenvironment: changes in protein expression in an in vitro co-culture system

Figure 1

Phase contrast microscopy. (A) Co-culture of MG-63 cells and HFs grown for 4 days. Cord-like formations are well detected (*). HFs worked as a climbing frame on which the MG-63 cells (arrow) adhered and proliferated. Single HFs were also observed (arrowhead). (B) phase contrast microscopy of the co-culture of MG-63 cells and HFs grown for 7 days. Thicker cord-like formations were detected (*). (C) phase contrast microscopy of the co-culture of MG-63 cells grown separately in monolayers for 24 h. (D) phase contrast microscopy of HFs grown in monolayers for 24 h. (E) phase contrast microscopy of MG-63 cells grown in monolayers for 7 days. (F) phase contrast microscopy of HFs grown in monolayers for 7 days. Magnification for all the images was 100X. Aperture number is 0.25.

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