Migrating astrocytoma cells exhibit serum-dependent intracellular Ca2+oscillations at varying frequencies. A: Cells plated on Matrigel-coated glass-coverslips were loaded with Oregon green and observed using a confocal microscope. Representative traces of individual cells show a varying number of spontaneous intracellular Ca2+ transients. Cells were grouped according to the number of intracellular Ca2+ transients displayed during the 15 minutes acquisition period. B: The percentage of cells displaying 0, 1–9, 10–19 and >20 intracellular Ca2+ transients during 15 minutes, in the presence or absence of 10% FCS. C: The percentage intracellular Ca2+ transients during 15 minutes, in the presence or absence of the functional antibody P4C10 directed against β1 integrins. Data are mean ± SEM of 4 independent experiments, n indicate the number of cells analyzed per conditions.