Figure 4

Glutamate-induced increase in [Ca²⁺] _i in astrocytoma cells. A: Quantification of changes in [Ca²⁺]_i as measured microfluorimetrically in Fura-2 loaded cells. Typical individual responses observed after addition of 300 μM glutamate are shown. In a large number of cells, glutamate elicited single Ca²⁺ spike while a second spike can be observed in 10-20% of the cells analyzed. B: Glutamate induced a concentration-dependent increase in [Ca²⁺]_i. Data are mean ± SEM of 4 independent experiments.