Figure 5

Inhibition of glutamate reuptake induced increase in Ca²⁺oscillations frequency. Fluo-3 loaded cells were allowed to migrate on Matrigel-coated glass coverslips and changes in fluorescence were measured at 522 nm using confocal microscopy before and after addition of 100 μM L-THA. Top: Typical individual response of spontaneous Ca²⁺ oscillations is presented. Note the increase in spontaneous Ca²⁺ oscillations frequency after addition of L-THA. Bottom: In non oscillating, quiescent cells, addition of L-THA induced Ca²⁺ oscillations as measured by changes in fluo-3 fluorescence. Data are mean ± SEM of 4 independent experiments with 67 cells analyzed per condition.