Figure 3
From: Caveolin-1 induces lamellipodia formation via an Akt-dependent pathway
Effect of Akt inhibitor on lamellipodia formation. (A) Cav-1 overexpressed (H23/Cav-1) and control (H23/Ctrl) H23 cells were treated with Akt inhibitor (LY294002) at the concentration of 10 μM. Expression level of total Akt and activated Akt was determined. The blots were reprobed with β-actin to confirm equal loading. (B) Lamellipodia in control (H23/Ctrl) and Cav-1 overexpressing (H23/Cav-1) cells were indicated by arrow. Data were plotted as relative number of lamellipodia per cell in each field and represented the means ± SD (n = 4). *, p < 0.05 vs. control transfected (H23/Ctrl) cells at each treatment.