Figure 4

Caveolin-1 regulates lamellipodia via Akt-dependent mechanism. Cav-1 overexpressed (H23/Cav-1) cells were transfected with siRNA-Akt. (A) Akt and activated Akt levels were analyzed by western blot analysis. The immunoblot signals were quantified by densitometry, and mean data from independent experiments were normalized to the results. The bars are the means ± SD (n = 4). *, p < 0.05 vs. control transfected (H23/Cav-1) cells. (B) Migratory activity of H23/Cav-1 transfected with siRNA-Akt was determined by wound healing assay. Data represent the means ± SD (n = 4). *, p < 0.05 vs. control transfected (H23/Cav-1) cells. (C) Migratory activity of Cav-1 overexpressing cells transfected with either siAkt or control plasmid were investigated by transwell migration assay as described. (D) Lamellipodia in the cells were investigated and indicated by arrow as described.