Figure 1

Metformin inhibits Ishikawa cell growth and induces cell death. (A) WST-8 assays were used to measure the viability of Ishikawa cells after treatment with metformin (0, 0.1, 1, 5, 10, or 20 mM) for 24 or 48 h. (B) Colony formation assays were used to measure the colonogenicity of Ishikawa cells after metformin treatment. The number of untreated cells was set as 100%. The data are mean values from triplicate experiments. *p < 0.05, **p < 0.01, one way ANOVA, post hoc comparisons, Tukey’s test. Columns, mean; error bars, SD.