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Figure 2 | Cancer Cell International

Figure 2

From: Metformin impairs growth of endometrial cancer cells via cell cycle arrest and concomitant autophagy and apoptosis

Figure 2

Metformin induces cell cycle arrest. (A) Ishikawa cells were treated with metformin (0, 5, or 10 mM) for 48 h. After fixation, the cell cycle was analyzed by flow cytometry. Quantitative analysis of percentage gated cells at the G0/G1, S, and G2/M phases are shown. Data are shown as mean ± SD of three independent replicate measurements. *p < 0.05 and **p < 0.01 vs. untreated cells. (B) Ishikawa cells were treated with metformin (0, 0.1, 1, 5, or 10 mM) for 48 h. Cell lysates were separated by SDS-PAGE and analyzed on western blots with the indicated antibodies. Actin was used as a loading control. One representative experiment of three experiments is shown. (C and D) Densitometric quantitation of p21/actin and p53/actin protein expression levels is shown as fold changes. One representative experiment of three is shown. *p < 0.05 and **p < 0.01 vs. untreated cells.

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