Skip to main content
Figure 3 | Cancer Cell International

Figure 3

From: Metformin impairs growth of endometrial cancer cells via cell cycle arrest and concomitant autophagy and apoptosis

Figure 3

Metformin induces apoptosis in Ishikawa cells. (A) Ishikawa cells were treated with metformin (0, 5, or 10 mM) for 48 h. Cells were harvested and stained with annexin V-FITC and 7-AAD, and cell apoptosis was analyzed using flow cytometry. *p < 0.05 and **p < 0.01 vs. untreated cells. (B) Ishikawa cells were treated with metformin (0, 5, or 10 mM) for 48 h, stained with JC-1, incubated, and analyzed using flow cytometry. *p < 0.05 and **p < 0.01 vs. untreated cells. (C and D) Ishikawa cells were treated with metformin (0, 0.1, 1, 5, or 10 mM) for 48 h. Cell lysates were separated by SDS-PAGE and analyzed on western blots probed with the indicated antibodies. Actin was used as a loading control. Densitometric quantitation of Bax/Bcl-2 protein expression ratios are shown as fold changes. One representative experiment of three is shown. (E) Ishikawa cells were incubated with metformin (0, 5, or 10 mM) for 48 h, followed by assays of caspase-3/7, -8, and -9 activity. *p < 0.05 and **p < 0.01 vs. untreated cells.

Back to article page