Figure 3

Effects of TLR activation on proliferation of human prostate cancer cell lines. The effects of LPS (A) and LTA (C) on proliferation of prostate cancer cell lines were examined. Cells were treated with a wide concentration range of LPS or LTA and their proliferation was measured by XTT assay in reference to vehicle-treated cells. To rule out the possibility of contamination of LPS with other TLR ligands, LPS was neutralized by Polymyxin B and the level proliferation in DU145 cells was assessed either in the presence or absence of soluble CD14 (B). Each bar represents the mean ± SD of three independent experiments and each test was done in triplicate. In each cell line, the data were compared to vehicle-treated (control) cells. c: p < 0.001, a: p < 0.05.