The same and not the same: heterogeneous functional activation of prostate tumor cells by TLR ligation

Table 1 Sequence, amplicon size and annealing temperatures of primers used in this study

	Forward (5′–3′)	Reverse (5′–3′)	Product size (bp)	Annealing Temp(°C)
TLR1	CTATACACCAAGTTGTCAGC	GTCTCCAACTCAGTAAGGTG	220	60
TLR2	GTACCTGTGGGGCTCATTGT	CTGCCCTTGCAGATACCATT	191	62
TLR3	GATCTGTCTCATAATGGCTTG	GACAGATTCCGAATGCTTGTG	305	60
TLR4	GAGCTTTAATCCCCTGAGGCA	GATTGGATAAGATTGTGAGCC	306	54.8
TLR5	CTAGCTCCTAATCCTGATG	CCATGTGAAGTCTTTGCTGC	438	59
TLR6	AGGTGCCTCCATTATCCTCA	GAATCCATTTGGGAAAGCAG	211	59
TLR7	CATGCTCTGCTCTCTTCAACC	CGATCACATGGTTCTTTGGA	201	59
TLR8	GCCAGCGAGTCTCACTGAACT	GCCAGGGCAGCCAACATA	558	61
TLR9	TTCCCTGTAGCTGCTGTCC	ACAGCCAGTTGCAGTTCACC	207	58
TLR10	GGCCAGAAACTGTGGTCAAT	CTGCATCCAGGGAGATCAGT	199	61
CD14	AGAGTTCACAAGTGTGAAGC	CCTTGACCGTGTCAGCATA	373	58
MyD88	GACCCAGCATTGAGGAGGATTG	GCTTCTGATGGGCACCTGGAGAG	435	60
βactine	GTGGGGCGCCCCAGGCACCA	CTCCTTAATGTCACGCACGATTTC	538	60

ISSN: 1475-2867