Figure 1
Construction of expression vectors. (A) A schematic representation of the recombinant plasmids. The pcDNA3.1-rps4 vector was constructed by inserting the rps4 gene fragment into Xba I and Kpn I sites of pcDNA3.1/Hygro (-) between the PCMV (cytomegalovirus promoter) sequence and the BGHpA (bovine growth hormone polyadenylation) sequence. The pcDNA3.1-mrp4 vector was constructed by inserting the Dd-mrp4 gene into pcDNA3.1/Hygro (-), in the same way as pcDNA3.1-rps4 vector. (B) Identification of the pcDNA3.1-rps4 vector by using restriction enzyme digestion and agarose gel electrophoresis. Lane C, non-digested; lanes 1-3, expression vector digested with Xba I, Kpn I, and Xba I &Kpn I, respectively. (C) Identification of the pcDNA3.1-mrp4 vector by using restriction enzyme digestion and agarose gel electrophoresis. The lanes are the same as those shown in (B).