Figure 3

Effects of enforced Dd-mrp4 expression on the proliferation of several human primary cultured cells. HBMECs, HUVECs, HCV and hNHeps cells were seeded in a 6-well plate and transfected with the pcDNA3.1, pcDNA3.1-rps4 (negative control), or pcDNA3.1-mrp4 vector for 6 hrs. After 42 hrs of incubation at 37°C, the cells were treated with WST-8 solution for 2 hrs, and their viability was monitored as described in Methods. The results represent the mean ± SD in three independent experiments. ns; not significant.