Figure 2

MiR-744 restoration inhibit the viability of HCC cells. (A) miR-744 expression was quantified by qRT-PCR analysis. miR-744 mimics restorated miR-744 expression in both HepG2 and SMMC-7721 cells. (B) Proliferation assay of HCC cells in response to miR-744 restoration. HepG2 or SMMC-7721 cells were seeded into 96-well plates and incubated in the presence of miR-744 mimics or negative control oligonucleotides. Cell proliferationassay was done after culturing for 72 h. (C, D) Cell-cycle distribution was analyzed by FACS analysis. HepG2 (C) and SMMC-7721 (D) cells were transfected with miR-744 mimics or negative control oligonucleotides. Nocodazole (25 ng/ml) was added 24 h after transfection for another 16 h, then the supernatant was replaced by fresh medium for 6 h. The data were subjected to Student's t-test. *p < 0.05, **p < 0.01. NC, negative control oligonucleotides.