Effect of red wine on Akt and ERK. Total cell lysates were prepared from A549 cells that were serum-deprived for 24 h then treated with or without 5% wine for 24 h. Stimulation with 100 ng/ml EGF was performed for 10 min before cell lysis (C, D). Cell lysates (20 μg) were resolved by SDS-PAGE and immunoblotted with anti-phospho, or anti-total- Akt antibody (A, C) or anti-phospho, or anti-total- Erk antibody (B, D). Representative immunoblots of 3 independent experiments are shown. Immunoblots were scanned to quantitate the density of the bands and the results in the bar graphs are the mean ± SE of 3 independent experiments. All values are arbitrary densitometry units expressed relative to untreated control. *P < 0.05, **P < 0.01 vs. control, #p < 0.05 vs. EGF.