Effect of red wine on p53. Serum-deprived (24 h) A549 cells were treated with or without 5% red wine for 24 h. Total cell lysates were prepared and 20 μg of protein from each sample were resolved by SDS-PAGE and immunoblotted with anti-phospho, or anti-total- p53 antibody. A representative immunoblot is shown. Immunoblots were scanned to quantitate the density of the bands and the results in the bar graphs are the mean ± SE of 3 independent experiments. All values are arbitrary densitometry units expressed relative to untreated control.