Effect of white wine on Akt in A549 NSCLC cells. Total cell lysates were prepared from A549 cells that were serum-deprived for 24 h then treated with or without 2% or 5% white wine (Riesling) for 24 h. Cell lysates (25 μg) were resolved by SDS-PAGE and immunoblotted with anti-phospho or anti-total- Akt antibody. Representative immunoblots of 2 independent experiments are shown. Immunoblots were scanned to quantitate the density of the bands and the results in the bar graphs are mean ± SE. All values are arbitrary densitometry units expressed relative to untreated control.