Figure 3

Effect of GR on the proliferation of MDA-MB 231 and MCF-7 BIBR 1532 treated cells. The cells were seeded at a very low density in a 25 cm² flask and grown in DMEM culture medium containing 10% FBS and 1% Pen/Strep at different glucose concentrations with or without 2.5 μM of BIBR 1532. The medium was replaced every two days, and fresh telomerase inhibitor was added each time. At the end of each week, the cells were split and counted at least three times using bromophenol blue and a hemocytometer. a and b show the effect of GR and treatment with 2.5 μM BIBR 1532 on MDA-MB 231 and MCF-7 cells based on the obtained cell number after each count compared to the number seeded cells. Bars represent values from at least three independent experiments (*p < 0.05, *p < 0.01).