Figure 5

Proliferation test of MDA-MB 231, MCF-7 and of saos-2 cells. Cells grown in DMEM with different glucose concentrations were equally seeded in 96-well plates at a density of 10⁴ cells/well. Twenty-four hours later, the cells were treated with different concentrations of BIBR 1532 for 48 hours. At the end of treatment, each well received 10 μl of tetrazolium salt and was incubated for 60 minutes at 37°C. The formation of formazan dye was assessed using an ELISA reader at 450 nm. a and b represent the dose response curves of MDA-MB 231 and MCF-7 to BIBR 1532, respectively. c represents the effect of GR, while d represents the effect of BIBR 1532 on the mitochondrial metabolism of saos-2 cell line. The O.D. of each condition is a mean of 12 wells. Bars represent values from at least three independent experiments (*P < 0.05, **P < 0.01).