Figure 6

Effect of telomerase siRNA on cell viability and mitochondrial metabolism. MDA-MB 231 (a) and MCF-7 (b) cells grown in DMEM with different glucose concentrations were equally seeded at a density of 2 × 104 in a 96-well plate 10 min after 20 nM SiRNA transfection. Seventy-two hours later, each well received 10 μl of WST-1 and was incubated for 60 minutes at 37°C. The formation of formazan dye was assessed using an ELISA reader at 450 nm. The O.D. of each condition is a mean of 12 well. Bars represent values from at least three independent experiments. (*P < 0.05, **P < 0.01).