Figure 7
Effect of high BIBR 1532 concentration on cell apoptosis. MDA-MB 231 cells grown at different glucose concentrations were seeded in 6-well plates at a density of 2 × 105 cells per well. After adherence, the cells were treated with 50 μM BIBR 1532 for 48 hours. After treatment, the culture media was discarded, and the cells were harvested and lysed as mentioned in materials and methods to further quantify the caspase-3 level via ELISA and measure the protein concentration. Bars represent values from at least three independent experiments (*p < 0.05).