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Figure 5 | Cancer Cell International

Figure 5

From: The RelB alternative NF-kappaB subunit promotes autophagy in 22Rv1 prostate cancer cells in vitro and affects mouse xenograft tumor growth in vivo

Figure 5

RelB effects on cell death of 22Rv1 cells. A) Illustration of 22Rv1-derived cells cultured in suspension on poly-HEMA pre-coated plates. Representative fields at 200X magnificence. B) Cell death evaluation in suspension cell culture of 22Rv1-derived cells. Cells were cultured 7 days in 96-well plates pre-coated or not with poly-HEMA. Cell death was evaluated by bioluminescence using the CytoTOX-Glo cytotoxicity assay. Data represent the ratio of basal cell death/total cell death after complete lysis using digitonin reagent. Cell death in suspension culture conditions are illustrated in black bars and adherence control cell culture conditions in empty bars. The variation was significant at p < 0.05 (Mann- Whitney U test). (*) illustrates significant variations with both GFP control populations. Error bars represent the standard error of the mean. C) Immunoblot analyses of cell death from whole protein extracts of 22Rv1-derived cells following 7-day suspension cell culture RelB status, PARP cleavage (apoptosis) and LC3-I to LC3-II conversion (autophagy) were evaluated. Actin was used as loading control.

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