Knocking down PTOP protein in different groups. Western blot showed the PTOP knocking down expression in different groups. Compare with the MDA-MB-435 s cell, the expression of 60/2 PTOP RNAi and 60/6 PTOP RNAi cells were significant decreased. The bar graph showed the semiquantitative analysis of PTOP knocking down expression. Data represented means ± SD, *p < 0.05 was considered significant.