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Figure 2 | Cancer Cell International

Figure 2

From: Real-time RT-PCR analysis of mRNA decay: half-life of Beta-actin mRNA in human leukemia CCRF-CEM and Nalm-6 cell lines

Figure 2

Real-Time RT-PCR quantification of β-actin mRNA. Standard curve showing amplification efficiencies of ten-fold serial dilutions of the pBactin-231 template. Real-Time PCR amplifications were performed using β-actin specific primers BA67 and BA68. The calculated Ct values were plotted versus the log of the initial amount of pBactin-231 molecules (104 to 109) to generate the standard curve. Squares and circles represent β-actin standards and mRNA samples, respectively.

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