Table 1 Results of 51Cr release assays of labeled (H) vascular ECs, MΦ, AIDS-KS10 and AIDS-KS21 cells all at 50:1 E:H ratio and with: NMS-1 at 1:400 and 1:100 dilutions (columns 2 & 3), mAb RH1-38 also at 1:400 and 1:100 dilutions (columns 4 & 5), neither RH1-38 nor NMS-1 (column 6). Values in brackets indicate percentage (%) inhibition of NMS-1 (1:400) lysis.
E:Ab | NMS-1 (1:400) | NMS-1 (1:100) | RH1-38 (1:400) | RH1-38 (1:400) | NoRH1-38/NMS-1 |
|---|---|---|---|---|---|
Cell Type (H) | Experiment # 1 Percent (%) Lysis ± SEM | ||||
I. MΦ | 60 ± 4.1 | 50 ± 4.9 | 19 ± 0.9(68) | 1 ± 0.8(98) | 60 ± 3.9 |
II. Vascular ECs | 67 ± 2.8 | 68 ± 2.7 | 30 ± 0.6(55) | 5 ± 0.3(93) | 66 ± 1.3 |
III. AIDS-KS10 | 85 ± 0.3 | 75 ± 1.3 | 33 ± 0.7(61) | 8 ± 0.6(91) | 80 ± 2.4 |
IV. AIDS-KS21 | 81 ± 4.3 | 80 ± 2.2 | 75 ± 2.0 (7) | 74 ± 4.5(9) | 81 ± 4.9 |
|  | Experiment # 2 Percent (%) Lysis ± SEM | ||||
I. MΦ | 40 ± 1.7 | 39 ± 2.8 | 12 ± 1.4(70) | 4 ± 1.0(90) | 44 ± 1.0 |
II. Vascular ECs | 80 ± 1.2 | 75 ± 1.5 | 38 ± 0.9(53) | 10 ± 0.9(88) | 77 ± 0.3 |
III. AIDS-KS10 | 76 ± 2.0 | 74 ± 1.2 | 30 ± 0.3(60) | 9 ± 0.6(88) | 75 ± 2.0 |
IV. AIDS-KS21 | 55 ± 1.2 | 54 ± 1.3 | 49 ± 0.9(11) | 54 ± 2.3 (2) | 52 ± 2.1 |