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Figure 3 | Cancer Cell International

Figure 3

From: A phosphorylation defective retinoic acid receptor mutant mimics the effects of retinoic acid on EGFR mediated AP-1 expression and cancer cell proliferation

Figure 3

The S77A mutant is not phosphorylated on serine in SCC25 cells. Cells were stably transfected with HA tagged RARα (HA-RAR1,2), S77A mutant (HA-S77A1,2), or G418 resistance (neo) expression vectors. HA tagged RARα proteins were immunoprecipitated from cellular lysates using anti-HA antibody (IP HA). Blots were incubated with anti-phosphoserine antibodies (anti-pSer) to determine relative levels of RARα phosphorylation on serine residues. Blots were stripped and incubated with anti-RARα antibody to confirm the identity of the anti-HA immunoprecipitates. Blots were incubated with anti-HA antibody to ensure equal amounts of immunoprecipitated protein in each lane. These experiments were performed three times using different clones with similar results. Representative blots are shown.

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Cancer Cell International

ISSN: 1475-2867

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