Figure 3
From: Protein kinase ERK contributes to differential responsiveness of human myeloma cell lines to IFNα
Activation state of ERK in Sko-007 and U266 cells in the absence or presence of IFNα. 2 × 106 Sko-007 or U266 cells were left untreated or treated with IFNα (100 U/ml) for 48 h and lyzed in cell lysis buffer. Then cytoplasmic proteins were extracted and the expressions of phospho-ERK and ERK were determined by immunoblot assay as similar as in Fig. 2 except using anti-ERK or phospho-ERK antibodies.