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Table 1 Expression of IL-6R and gp130 on the surface of Sko-007 and U266 cells in the absence or presence of IFNα.

From: Protein kinase ERK contributes to differential responsiveness of human myeloma cell lines to IFNα

  IFNα (-) IFNα (+)
  % mean % mean
IL-6R 13.00 ± 1.21 20.57 ± 2.74 10.11 ± 0.96 19.80 ± 0.47
Sko-007     
Gp130 99.06 ± 1.01 60.68 ± 1.70 65.77 ± 3.19* 34.21 ± 1.31*
IL-6R 1.50 ± 0.33 50.44 ± 2.09 0.92 ± 0.36 50.09 ± 4.28
U266     
Gp130 8.66 ± 0.64 29.19 ± 2.13 9.33 ± 1.05 26.66 ± 0.63
  1. 2 × 105 or cells were treated with or without IFNα (100 U/ml) for 48 h and then subsequently incubated with anti-IL-6R or anti-gp130 antibodies for 30 min and with the relevant FITC-labeled second antibodies for 20 min. Cell samples were harvested and subjected to FACS analysis equipped with ModFitLT software. ''mean'' represents the average strength of fluorescence. ''%'' represents the percentage of the cells expressing target antigen (positive cells). The p values were determined by paired t-test. *significantly different (p < 0.01) from the corresponding values in cultures without IFNα.