Protein kinase ERK contributes to differential responsiveness of human myeloma cell lines to IFNα

Table 1 Expression of IL-6R and gp130 on the surface of Sko-007 and U266 cells in the absence or presence of IFNα.

2 × 10⁵ or cells were treated with or without IFNα (100 U/ml) for 48 h and then subsequently incubated with anti-IL-6R or anti-gp130 antibodies for 30 min and with the relevant FITC-labeled second antibodies for 20 min. Cell samples were harvested and subjected to FACS analysis equipped with ModFitLT software. ''mean'' represents the average strength of fluorescence. ''%'' represents the percentage of the cells expressing target antigen (positive cells). The p values were determined by paired t-test. *significantly different (p < 0.01) from the corresponding values in cultures without IFNα.