| IFNα (-) | IFNα (+) |
---|
| % | mean | % | mean |
---|
IL-6R | 13.00 ± 1.21 | 20.57 ± 2.74 | 10.11 ± 0.96 | 19.80 ± 0.47 |
Sko-007 | | | | |
Gp130 | 99.06 ± 1.01 | 60.68 ± 1.70 | 65.77 ± 3.19* | 34.21 ± 1.31* |
IL-6R | 1.50 ± 0.33 | 50.44 ± 2.09 | 0.92 ± 0.36 | 50.09 ± 4.28 |
U266 | | | | |
Gp130 | 8.66 ± 0.64 | 29.19 ± 2.13 | 9.33 ± 1.05 | 26.66 ± 0.63 |
- 2 × 105 or cells were treated with or without IFNα (100 U/ml) for 48 h and then subsequently incubated with anti-IL-6R or anti-gp130 antibodies for 30 min and with the relevant FITC-labeled second antibodies for 20 min. Cell samples were harvested and subjected to FACS analysis equipped with ModFitLT software. ''mean'' represents the average strength of fluorescence. ''%'' represents the percentage of the cells expressing target antigen (positive cells). The p values were determined by paired t-test. *significantly different (p < 0.01) from the corresponding values in cultures without IFNα.