|
IFNα (-)
|
IFNα (+)
|
---|
|
%
|
mean
|
%
|
mean
|
---|
IL-6R
|
13.00 ± 1.21
|
20.57 ± 2.74
|
10.11 ± 0.96
|
19.80 ± 0.47
|
Sko-007
| | | | |
Gp130
|
99.06 ± 1.01
|
60.68 ± 1.70
|
65.77 ± 3.19*
|
34.21 ± 1.31*
|
IL-6R
|
1.50 ± 0.33
|
50.44 ± 2.09
|
0.92 ± 0.36
|
50.09 ± 4.28
|
U266
| | | | |
Gp130
|
8.66 ± 0.64
|
29.19 ± 2.13
|
9.33 ± 1.05
|
26.66 ± 0.63
|
- 2 × 105 or cells were treated with or without IFNα (100 U/ml) for 48 h and then subsequently incubated with anti-IL-6R or anti-gp130 antibodies for 30 min and with the relevant FITC-labeled second antibodies for 20 min. Cell samples were harvested and subjected to FACS analysis equipped with ModFitLT software. ''mean'' represents the average strength of fluorescence. ''%'' represents the percentage of the cells expressing target antigen (positive cells). The p values were determined by paired t-test. *significantly different (p < 0.01) from the corresponding values in cultures without IFNα.