Figure 3

Effects of IL-6 and wortmannin on the expression of apoptosis-related proteins in 7TD1 cells. (A) 7TD1 cells were starved in IL-6-free medium for 6 h. Then the cells were cultured in the presence of 1 ng/ml IL-6 for various periods as indicated. At the end of the incubation period, whole-cell extracts were prepared and subjected to Western-blotting assay. (B) 7TD1 cells were starved in IL-6-free medium for 6 h. Then the cells were pretreated with wortmannin or DMSO of equal volume for 30 min at 37°C before IL-6 was added into the medium. The cells were cultured for 1 h in the presence of IL-6. After the cells were collected and washed, whole-cell extracts were prepared and subjected to Western-blotting assay.