Figure 5
Effect of an IGF-RI blocking antibody on the induction of p21 Cip1/Waf1 Serum-starved MCF-7S cells were grown in 6-wells plates, and were preincubated for 1 hour with 1 μg/ml of αIR3 antibody before stimulation with mitogens. They were given no mitogens (-), 20 ng/ml of IGF-I (I(20)), or 100 ng/ml of TPA (TPA) and were lysed after 4 h. Levels of p21Cip1/Waf1 were analyzed by Western blotting.