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Figure 10 | Cancer Cell International

Figure 10

From: Inducible expression of catalytically active type 1 serine/threonine protein phosphatase in a human carcinoma cell line

Figure 10

Isoform-specific antibody detection of PP1 by western blotting induced and uninduced LLWO2F whole-cell lysates. Different amounts (5 ug for the first and third lanes, 10 ug for the second and fourth lanes) of lysate from induced and uninduced cells were separated by SDS-PAGE in parallel and then immunoblotted using PP1α-, PP1δ-, and PP1γ1-specific antibody. Results illustrate downregulation of endogenous PP1α in response to increasing amounts of induced 6His-HA-PP1α, while the levels of PP1δ and PP1γ1 remain relatively constant. Lane C – control lane of isoform-specific antibody immunoprecipitation of respective isoforms from 50 ug of uninduced cell lysate.

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