Figure 4From: Inducible expression of catalytically active type 1 serine/threonine protein phosphatase in a human carcinoma cell lineCoprecipitation of 6His-HA-PP1α with GST-PNUTS. Doxycycline-induced LLWO2F cell lysates were mixed with either GST or GST-PNUTS bound to glutathione-Sepharose beads, washed, and the bound proteins separated by SDS-PAGE and then immunoblotted with antibody to hemagglutinin. Position of 6His-HA-PP1α is indicated to the left of the panel, and is present only in the GST-PNUTS lane. The band above 6His-HA-PP1α, which is also present in the GST-alone lane, results from non-specific reactivity with the secondary antibody (horse-radish peroxidase-conjugated anti-IgG) used for chemiluminescent detectionBack to article page