High dietary level of synthetic vitamin E on lipid peroxidation, membrane fatty acid composition and cytotoxicity in breast cancer xenograft and in mouse host tissue

Table 1 The percent of total fatty acid composition of linoleic acid (LA, n6 18-2), arachidonic acid (AA, n6 20-4), and docosahexaenoic acid (DHA, n3 22-6) in microsomal and in mitochondrial fractions of tumor, of liver and of colon following a two week dietary intervention of AIN·76 food formula without (-) and with (+) supplementation with synthetic vitamin E (2000 IU/kg dry food). Mean ± SD and number of mice (n) are listed.

Vitamin E supplement	Fraction	n	LA, 18-2^a	AA, 20-4	DHA, 22-6^b
Tumor
-	Micro	4	8.7 ± 5.0	7.4 ± 12	0.3 ± 0.3
+	"	5	1.9 ± 2.1	7.9 ± 4.2	1.3 ± 1.3
-	Mito	4	7.0 ± 5.9	9.6 ± 2.8	0.2 ± 0.5
+	"	5	4.4 ± 5.9	5.8 ± 3.7	2.3 ± 4.9
Liver
-	Micro	4	12.9 ± 5.1	4.9 ± 1.8	1.0 ± 0.9
+	"	5	11.3 ± 1.7	5.4 ± 0.9	1.8 ± 0.3
-	Mito	4	14.2 ± 1.5	8.0 ± 0.9	0.5 ± 0.1
+	"	5	12.9 ± 1.2	6.1 ± 1.8	1.4 ± 0.5
Colon
-	Micro	3	5.3 ± 2.1	6.3 ± 2.2	0.3 ± 0.3
+	"	5	4.6 ± 1.2	4.1 ± 1.3	2.4 ± 1.1
-	Mito	3	9.4 ± 2.1	2.9 ± 0.2	0.8 ± 0.5
+	"	5	6.3 ± 1.3	3.7 ± 1.6	1.8 ± 1.0

^a6 of 6 means lower with vitamin E supplement (p < 0.01 by Fisher's exact test) ^b6 of 6 means higher with vitamin E supplement (p < 0.01 by Fisher's exact test)

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