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Table 1 Isoform specific antibodies used to detect the isoforms of Na, K-ATPase in canine prostatic tissue by immunohistochemical analysis.

From: Epithelial Na, K-ATPase expression is down-regulated in canine prostate cancer; a possible consequence of metabolic transformation in the process of prostate malignancy

Antibody Isoform Specificity Working Dilution Source References
α5§ Recognizes all Neat Supernatant D. M. Fambrough [32]
Pan α (monoclonal) known α isoforms    
mAb9A7 Recognizes all 1:100 M. Takahashi [21]
Pan α (monoclonal) known α isoforms    
α620 (polyclonal) α1 specific 1:200 M.J. Caplan [33]
McB2 (monoclonal) α2 specific Neat Supernatant K.J. Sweadner [34]
XVIF9G10 (monoclonal) α3 specific Neat Supernatant K.P. Campbell [35]
SpETb1 (polyclonal) β1 specific 1:200 P. Martín-Vasallo [36]
SpETb2 (polyclonal) β2 specific 1:200 P. Martín-Vasallo [36]
γC33 (polyclonal) γ specific 1:200 S.J.D. Karlish [19]
  1. § The α5 monoclonal broadly recognizes Na+, K+-ATPase α subunits of avian, mammalian and insect species. There are 4 known α isoforms and the designation " α5" does not indicate the existence of a fifth Na+, K+-ATPase α isoform. The α5 antibody developed by D. Fambrough was also obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242, U.S.A. The monoclonal antibody XVIF9G10 developed by K.P. Campbell is commercially available from Affinity Bioreagents.