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Table 2 Dependence of DNA fragmentation is shown on drugs and cell lines using APO-BRDU endlabelling technique for various antinucleoplastic agents (VP16 etoposide, 9-amino-campothecin, cisplatin) at various times (8 and 24 hours).

From: Chemosensitivity assay in mice prostate tumor: Preliminary report of flow cytometry, DNA fragmentation, ion ratiometric methods of anti-neoplastic drug monitoring

Drug exposure with cell lines APO-BRDU Endlabelling Fluorescence (n = 5)
Exposure(in hours) 0 8 24 44
Taxotere + DU145 - 1.5 3 1.8
Taxotere + PC3 - 1.5 2 3.5
VP 16 + DU145 - 3.8 9 4
VP 16 + PC3 - 2 4 11
Campothecin + DU145 - 0.3 34 -
Cisplatin + DU145 - 0.3 54 -
  1. Mean fluorescent intensity (per 10 × 103 cells) values are shown at different experimental conditions (see Figure 3A). It illustrates fluorescent intensities at standard tissue culture doses: i. minimal response at 8 hours; ii. a range of responses for a given cell lines (highest DU145 response with VP16 while negligible with cisplatin; and iii. for same drug and dose (VP16 at 10 μg/ml). Notice the DU145 pronounced response greater than PC3 response.