Chemosensitivity assay in mice prostate tumor: Preliminary report of flow cytometry, DNA fragmentation, ion ratiometric methods of anti-neoplastic drug monitoring

Table 2 Dependence of DNA fragmentation is shown on drugs and cell lines using APO-BRDU endlabelling technique for various antinucleoplastic agents (VP16 etoposide, 9-amino-campothecin, cisplatin) at various times (8 and 24 hours).

Drug exposure with cell lines	APO-BRDU Endlabelling Fluorescence (n = 5)
Exposure(in hours)	0	8	24	44
Taxotere + DU145	-	1.5	3	1.8
Taxotere + PC3	-	1.5	2	3.5
VP 16 + DU145	-	3.8	9	4
VP 16 + PC3	-	2	4	11
Campothecin + DU145	-	0.3	34	-
Cisplatin + DU145	-	0.3	54	-

Mean fluorescent intensity (per 10 × 10³ cells) values are shown at different experimental conditions (see Figure 3A). It illustrates fluorescent intensities at standard tissue culture doses: i. minimal response at 8 hours; ii. a range of responses for a given cell lines (highest DU145 response with VP16 while negligible with cisplatin; and iii. for same drug and dose (VP16 at 10 μg/ml). Notice the DU145 pronounced response greater than PC3 response.

ISSN: 1475-2867