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Figure 6 | Cancer Cell International

Figure 6

From: Sulindac derivatives inhibit cell growth and induce apoptosis in primary cells from malignant peripheral nerve sheath tumors of NF1-patients

Figure 6

Activation of SAPK/JNK but not caspase-3 after treatment with Sulindac metabolites The activation of phospho-SAPK/JNK (dimer: 46 kD + 54 kD) reached the highest level after 24 h of treatment with Exisulind (Exi) and Sulindac Sulfide (s. s.), but no effect was seen on cleaved caspase-3 activation. DHA did not have any effect on neither JNK nor caspase-3 activation. Cell line S462 was grown in DMEM with 10% serum and treated at 80–90% confluency with 0.2% DMSO, 500 μM Exisulind (Exi) or 125 μM Sulindac Sulfide (s. s.) and blots were incubated with either phosphorylated SAPK/JNK or cleaved caspase-3 antibody. The positive control for caspase activation is a mouse MPNST cell line treated 7 h with 30 μM DHA.

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