Figure 7

HCC cell culture medium alone and in the presence of AAT studied by Western blot analysis. Cell culture supernatants were applied to 10% SDS-PAGE and immunoblotted with polyclonal antibody against AAT. M, molecular size markers (myosin-205 000, β-galactosidase-123 000, bovine serum albumin-79 000, carbonic anhydrase-45 700), lane 1-HCC cells alone, lane 2-HCC cells stimulated with AAT, lanes 3 and 4-HCC cells cultured in PMN-conditioned medium alone and supplemented with AAT, respectively lane 5-ATT done. The arrow indicates complexed AAT. The AAT profile shown in this figure represents one of three similar experiments.